Molecular cloning of infectious DNA from human papovavirus BK in Escherichia coli.
نویسندگان
چکیده
Recombinant DNA constructed from unit length BK virus DNA and from several defective viral forms was cloned in Escherichia coli HB101. The cloned unit length BK virus DNA retained its infectivity for human embryonic kidney cells, whereas the cloned defective DNA showed no infectivity. Restriction endonuclease digestion of cloned defective DNA and purified virus DNA indicated that many of the defective forms contain re-iterated sequences.
منابع مشابه
Cloning and sequencing of ompf Salmonella typhi Salmonella ompf gene in Escherichia coli Origami
Background and Aim: Salmonella Typhi belongs to the family Enterobacteriaceae, gram-negative bacilli and causes gastrointestinal diseases such as typhoid. This bacterium has a special structure and various genes, including the ompf gene (outer membrane protein). Recent studies have shown the possibility of using ompf in the development of a diagnostic tuberculosis vaccine. Therefore, the aim of...
متن کاملCloning and Expression of Human Gamma-Interferon cDNA in E. coli
Prior to the production of human gamma interferon using recombinant DNA technology, it had been producedmainly upon mitogenic induction of lymphocytes in very low amounts, which evidently hamperedits characterization and its medical applications. The recombinant gamma interferons produced in largerquantities in prokaryotic systems retain their biological activities, and can be...
متن کاملPhylogenetic typing and molecular detection of virulence factors of avian pathogenic Escherichia coli isolated from colibacillosis cases in Japanese quail
Colibacillosis caused by avian pathogenic Escherichia coli (APEC) is an economic threat to the poultry industry throughout the world. Some of the virulence genes may enhance the ability of E. coli isolates to grow in the tissues of broilers. The APEC strains are assigned to a few distinct phylogenetic groups. The purpose of the present study was to detect the virulence genes a...
متن کاملCloning and determination of biochemical properties of protective and broadly conserved vaccine antigens from the genome of extraintestinal pathogenic Escherichia coli into pET28a vector
Urinary tract infections are one of the most common infectious diseases that lead to significant health problems in the world. Urinary tract infections are referred to any infection in any part of the renal system. Uropathogenic Escherichia coli, Proteus mirabilis, and Klebsiella are main organisms that are involved in these infections. After identifying same protective and conserved virulence ...
متن کاملMolecular Cloning and Characterization of a Lipase from an Indigenous Bacillus pumilus
Cloning and sequencing of a lipase gene from an indigenous Bacillus pumilus, strain F3, revealed an open-reading frame of 648 nucleotides predicted to encode a protein of 215 residues. Sequence analysis showed that F3 lipase contained a signal peptide composed of 34 amino acids with an H domain of 18 residues. A tat-like motif was found in the signal peptide similar to some other Bacillus pumil...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- The Journal of general virology
دوره 55 Pt 1 شماره
صفحات -
تاریخ انتشار 1981